Journal of Diabetes and Metabolic Disorders 2006. 6(0):172-.

ASSOCIATION STUDY OF POLYMORPHISM OF THE PPAR-γ2 GENE AND OBESITY.
Hassan Mirzaei, Taghi Golmohammadi, Seyed Mohammad Akrami, Mahmood Doosti, Manochehr Nakhjavani, Ramin Heshmat, Parvin Amiri

Abstract


Background: Peroxisome Proliferators- Activated Receptor-Gamma2 (PPAR- γ2) is a nuclear receptor that regulates adipocyte differentiation, lipid metabolism and insulin sensitivity. The aim of this study was to evaluate the association of the Pro12Ala polymorphism at the PPAR- γ2 gene in Iranian population with obesity.


Methods: The genomic DNAs of the 156 subjects including obese and healthy isolated from EDTA whole blood. Pro12Ala polymorphism detected by Polymerase Chain Reaction - Restriction Fragment Length polymorphism (PCR-RFLP).


Results: In the obese group , one sample (1.3%) was as homozygote Ala/Ala genotype , 24 samples (30.8%) were Pro/Ala heterozygote and 53 samples (67.9%)as Pro/Pro genotype were identified . in the control group , one sample (1.3%) was as Ala/Ala genotype , 12 samples (15.4%) were Pro/Ala genotype and 65 samples (83.3%) were Pro/Pro genotype. allele frequencies of  Ala in obese subjects (qAla=%16.7)were significantly different from those in control subjects (qAla=%8.9).


Conclusion: Our results revealed that Pro12Ala polymorphism in PPAR- γ2 gene associated with obesity in the Iranian population and presence Ala allele cause to significantly higher BMI and lower fasting blood sugar.


Keywords


PPAR-γ2 gene, Obesity, Pro12Ala polymorphism,

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