Journal of Diabetes and Metabolic Disorders 2004. 3(0):66-.

POLYMORPHISM IN IFN GAMMA GENE HAS A NEGATIVE ASSOCIATION WITH TYPE 1 DIABETES MELLITUS IN IRANIAN PATIENTS.
Mohammad Hossein Niknam , Ali Rafinejad , Ali Akbar Amirzargar, Farideh Khosravi, Bagher Larijani

Abstract


Background: Type 1A Diabetes Mellitus (T1DM) is a chronic and progressive auto- immune disorder resulting from immune  mediated destruction of Langerhans islet beta cells.  The etiology of T1DM like the other autoimmune diseases is unknown and many factors are involved, Both humoral and cell-mediated immunity have a critical role in T1DM pathogenesis. The cytokines, the immunomodulatory peptides, are responsible for the immune cell recruitment and producing auto-antibodies by the immune effector cells. To evaluate the role of cytokines in sensitivity or resistance to T1DM, we have employed IFN gamma to determine their gene polymorphisms and their association with T1DM.


Methods: 30 patient suffering from T1DM and 40 normal control were studied simultaneously .PCR technique was used to characterize the polymorphisms of cytokine. Salting out method was performed for DNA isolation .The polymorphosime of IFN gamma gene was determined on position UTR+5664`5.The PCR products were evaluated by Gel Electerophoresis Technique.


Results: There was a significant difference between patient and control group in TT allele IFN gamma gene: p<0.05, RR: 0.39(0.22<RR<0.68), Since OR is <1 this correlation is a negative one .


Conclusion: In this study, we found a negative association between IFN gamma gene at position 5`UTR+5644 and T1DM in study group that means this allele may be a protective factor against T1DM.


Keywords


Type 1 Diabetes Mellitus, Gene Polymorphism, Cytokines,

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