Promoter resistin gene polymorphism in patients with type 2 diabetes and its influence on concerned metabolic phenotypes

Abstract

Introduction: Recently, it has been suggested that resistin, as an adipokine, links obesity with insulin resistance; moreover, the resistin gene polymorphisms, particularly in promoter region, is associated with serum resistin level and susceptibility to diabetes. This study investigates the association between resistin polymorphism at -420C/G and type2 diabetes (T2DM) in Iran.

Methods: This cross-sectional study was conducted on 47 type 2 diabetic patients and 66 non-diabetic controls. Blood sample was obtained from the subjects and after DNA extraction, the SNP analysis was performed via PCR-RFLP method. Then, the association between polymorphism in -420C/G and several clinical and biochemical characteristics including age of onset, fasting glucose and HbA1c were investigated.

Results: The frequency of CC genotype among diabetic patients (48.9%) as compared with healthy subjects (24.2%) was twofold.  The relative risk for -420 CC diabetic patients were 2.99(95% CI: 1.34-6.68, P= 0.009). The C allele frequency was higher among the diabetic patients compared with healthy controls, although this difference was not significant (P=0.13). Also, diabetic patients with CC genotype had the lowest age of onset and the highest fasting glucose and HbA1c among all studied patients (P≥0.05).

Conclusion: The results show that polymorphisms of promoter resistin gene are associated with type2 diabetes; nonetheless, CC genotype compared to GG and CG increases susceptibility to T2DM.

 Keywords: Polymorphism, Resistin, Type 2 diabetes